UHS-Dept

Research Activities

The postgraduate program at UHS aspires to higher education and research training in selected areas of Immunology. All students will carry out independent and original research under the supervision of faculty advisor.

Research Projects Completed (M.Phil Immunology- Basic Medical Sciences)

1. Association of Interleukin-4, IgE and LDL Oxidation in atherosclerosis

2. Polymorphism in methicillin resistance gene (mecA) of methicillin resistant Staphylococcus aureus isolates from Lahore and sero differences among different groups

3. Ability of different Staphlococcal aureus isolates to induce selected metabolites in human polymorphonuclaer leucocytes

4. Frequency of CD4+CD25+ T regulatory cells in males versus females

5. Levels of autoantibodies in healthy females and males

6. Enumeration of natural killer cells in diabetes mellitus patients treated with insulin and oral hypoglycemic

7. Enumeration of T regulatory cells in individuals with and without amalgam fillings in their teeth

8. Association of HLA-DR and HLA-DQ with bronchial asthma

9. Percentage of CD4+ CD25+ T regulatory cells in type 2 diabetic patients

10. Determination of serum level of IL-17 and vitamin D in asthmatic patients

11. Determination of salivary il-6 and il-17 in patients of calculus associated chronic periodontitis

12. Determination of salivary IgA ,TGF-beta, and IL-17 in patients of dental caries

13. Determination of anti endothelial cell, anti cardiolipin and anti glomerular antibodies in rheumatic heart disease patients

14. Determination of IFN-?, IL-2 and IL-10 levels in full term and preterm umbilical cord blood

15. Level of urinary CD14 in patients of acute coronary syndrome

16. Comparison of Anti cmDNA and Anti dsDNA Antibodies in Patients of Juvenile Systemic Lupus Erythematosus

17. Determination of serum NGAL level in patients of sle with and without nephritis

18. Determination of IL-10 gene polymorphism at promoter region (-592C/A and -1082A/G) and gene expression of IL-10 in patients of type 2 diabetes mellitus with and without nephropathy (Thesis Submitted)


Research Projects M.Sc MT (Completed)

1. Frequency of CD4+ and CD8+ T-lymphocytes in blood of tuberculosis patients

2. Level of interferon in the blood of tuberculosis patients

3. Determination of CRP and IL-6 in HCV positive and HCV negative patients of chronic renal failure on dialysis

4. Determination of rheumatoid factor and anti-cyclic citrulinated peptide antibodies in rheumatoid arthritis patients

5. Frequency of anti-neutrophil cytoplasmic antibody and anti-glomerular basement membrane antibody in glomerlonephritis patients

6. Detection of anti Helicobactor pylori antibodies in healthy subjects

7. Quantification of serum IgA concentration among patients of coeliac disease

8. Determination of anti-thyroid antibodies in patients of systemic lupus erythematosus

9. Prevalence of Antithyroid antibody in patients of hepatitis C infection of genotype 3a

10. Expression Analysis of Tumor Necrosis Factor Alpha (TNF-?) and Nuclear Factor Kappa Beta (NFkB) in patients of HCV genotype 3a

Research Projects Completed (M.Phil Immunology- Medical Laboratory Technology)

1. HLA DQB1 * * 0201 and DQB1 0301 alleles in patients of bronchial asthma and healthy controls visiting Gulab Devi Hospital Lahore

2. Interlukin-6 polymorphism(634C/G) in the promotor region and the progression of diabetic nephropathy in type 2 diabetes

3. Determination of Neutrophil Gelainase Associated Lipocalin (NGAL) protein in patients of systemic lupus erythematous with and without renal impairment

4. Association and polymorphism of SOCS-7 gene with interferon and insulin resistance and disease progression in chronic hepatitis C

5. Determination of Rheumatoid Factor of IgG, IgM and IgA isotypes in Rheumatoid Arthritis patients

6. Determination of antinuclear , antithyroid and anti islet cell antibodies in PCOS patients

7. Determination of TGF-B1 polymorphism at -509C/T promoter region in HCV and HCC patients

8. Polymorphism analysis of TLR 7 and TLR-9 genes in systemic lupus erythematosus

9. CD14 gene (C159T) polymorphism and its surface expression on monocytes in pulmonary TB patients

10. Determination of TLR gene polymorphism (rs4986791) and gene expression of IFN-? and TLR-4 in hepatitis C patients.

11. Determination of polymorphism +874, gene expression of IFN-? gene and serum level of IFN-? in patients of tuberculosis infections.

12. Enumeration of CD3-, CD56+, CD16+ and NKG2D+ Natural Killer Cells in Breast Cancer Patients

13. Enumeration of Circulating Tumor Cells in Breast Cancer Patients

14. Determination of anti-smooth muscles and anti-liver kidney microsomal type 1 autoantibodies and their association with HLA DR?1 (*04) allele in patients with chronic hepatitis C

15. Determination of anti-smooth muscles and anti-liver kidney microsomal type 1 autoantibodies and their association with HLA DR?1 (*04) allele in patients with chronic hepatitis C

Research Projects Ph.D (Completed)

  • Role of genetics and immune mechanisms in the pathogenesis of diabetic retinopathy
  • Genetic variation, expression of immune response genes and cell surface markers in Mycobacterium tuberculosis infection with and without rifampicin resistance.

Research Projects M.Phil Immunology- Basic Medical Sciences (Ongoing)

1. Determination of Polymorphism in Interferon-gamma Gene (rs+874) and levels of Interferon-gamma and Myeloperoxidase in patients with Oral Lichen Planus

2. Levels of IL-22 in diabetes mellites type 2 patients with and without nephropathy

3. Serum level of CD36 in pulmonary tuberculosis and health controls

4. Surface expression of CD40Ligand on T-cell and its level in pulmonary tuberculosis patients with or without treatment

5. Determination of anti-Pentraxin 3 and anti-dsDNA levels in patients of SLE with and without proteinuria and in healthy conrols.

6. Levels of Anti-Apolipoprotein A 1 autoantibodies in patients of Rheumatoid Arthritis and health control

Research Projects M.Phil Immunology- Medical Laboratory Technology (Ongoing)

  • Determination of PD-L1 gene polymorphism (A/L 8923) and gene expression analysis in oropharyngeal squamous cell carcinoma

. Determination of Th17 cells in TB patients

Research Projects Ph.D (Ongoing)

. HLA profile and immune markers in methotrexate responders and non-responders juvenile idiopathic arthritis patients

. Genetic variation and expression analysis of GIMAP4, IFN-? and interlukin-17 in patients of Tuberculosis with and without Drug resistance

. Identification and Characterization of Cancer Stem Cells in Head & Neck Squamous Cell Carcinoma with and without Human PapillomaVirus.

. Whole Genome Sequencing expression and gene variation analysis of SARS-CoV-2 and host genes(ACE-2, TLR, 2 and4) and immunological pattern in COVID-19 local patients.

HEC funded projects

1. Role of genetics and immune mechanisms in the pathogenesis of diabetic retinopathy. (HEC)

2. Gene expression analysis of cellular genes related to HCV induced pathogenesis. (HEC)

3. Inflammatory Cytokines, Allergy and Atherosclerosis

  1. Levels of autoantibodies in healthy females and males

5. Immunoglobulin light chain allelic inclusion in systemic lupus erythematosus and Rheumatoid Arthritis

6. Immunological, Viral and Genetic basis of COVID-19 in local patients (Rapid Research Grant) Word Bank

7. Molecular Characterization of Colistin and KPC bacterial resistance isolated from Hospitals and poultry industry & invitro efficacy of Silver Nano particles against MDR. (HEC/NRPU)

8. Molecular characterization of (NDM) producing microorganisms and efficacy of Manuka honey against NDM positive strains”. (HEC)/NRPU

9. Molecular and clinical characterization of cancer in Pakistan (Co-PI). (Thematic Project) .

Student Repository

S#

Year

Name of Student

Programme

Research Topic

Supervisor

Co-Supervisor

1

2005

Dr. Farhana Shazad

M. Phil Basic

Association of Interleukin-4, IgE and LDL Oxidation in atherosclerosis

Dr. Nadeem Afzal

2

2006

Dr. Gul Afshan

M. Phil Basic

Frequency of CD4+CD25+ T regulatory cells in males versus females

Dr. Abdul Ghaffar

3

Numan Javed

M. Phil Basic

Ability of different Staphlococcal aureus isolates to induce selected metabolites in human polymorphonuclaer leucocytes

Dr. Nadeem Afzal

4

Shahid Hussain

M. Sc

Level of interferon in the blood of tuberculosis patients

Dr. Nadeem Afzal

5

Khursheed Javaid

M. Sc

CD4+ and CD8+ T-lymphocytes in blood of tuberculosis patients

Dr. Nadeem Afzal

6

2007

Romeeza Tahir

M. Phil Basic

Polymorphism in methicillin resistance gene (mecA) of methicillin resistant Staphylococcus aureus isolates from Lahore and sero differences among different groups

Dr. Nadeem Afzal

7

Sarwar Abbas

M. Sc

Determination of CRP and IL-6 in HCV positive and HCV negative patients of chronic renal failure on dialysis

Dr. Nadeem Afzal

8

Sarah Kareem

M. Sc

Determination of rheumatoid factor and anti-cyclic citrulinated peptide antibodies in rheumatoid arthritis patients

Dr. Nadeem Afzal

9

Maria Arif

M. Sc

Frequency of anti-neutrophil cytoplasmic antibody and anti-glomerular basement membrane antibody in glomerlonephritis patients

Dr. Nadeem Afzal

10

2008

Amna Habib

M. Sc

Quantification of serum IgA concentration among patients of coeliac disease

Dr. Nadeem Afzal

11

Mehreen Ahmad

M. Sc

Detection of anti Helicobactor pylori antibodies in healthy subjects

Dr. Nadeem Afzal

12

2009

MuqaddasRiaz

M. Sc

Determination of anti-thyroid antibodies in patients of systemic lupus erythematosus

Dr. Nadeem Afzal

13

2010

Sana Tumori

M. Sc

Prevalence of Antithyroid antibody in patients of HCV of genotype 3a

Dr. Nadeem Afzal

14

Fatima Shabir

M. Sc

Expression Analysis of Tumor Necrosis Factor Alpha (TNF-?) and Nuclear Factor Kappa Beta (NFkB) in patients of HCV genotype 3a

Dr. Shah Jahan

15

2011

Faheem Shahzad

M. Phil Basic

Percentage of CD4+ CD25+ T regulatory cells in type 2 diabetic patients

Dr. Nadeem Afzal

16

Foqia Naz

M. Phil Basic

Determination of serum level of IL-17 and vitamin D in asthmatic patients

Dr. Nadeem Afzal

17

2012

Dr. Sarah Ashraf

M. Phil Basic

Levels of autoantibodies in healthy females and males

Dr. Nadeem Afzal

18

Dr. Rabia Tufail

M. Phil Basic

Enumeration of natural killer cells in diabetes mellitus patients treated with insulin and oral hypoglycemic

Dr. Nadeem Afzal

19

Dr. Zarghoona Jaffar

M. Phil Basic

Determination of IFN-g, IL-2 and IL-10 levels in full term and preterm umbilical cord blood

Dr. Nadeem Afzal

20

Dr. Fahad Khaliq

M. Phil Basic

Enumeration of T regulatory cells in individuals with and without amalgam fillings in their teeth

Dr. Nadeem Afzal

21

Khursheed Javaid

M. Phil Basic

Association of HLA-DR and HLA-DQ with bronchial asthma

Dr. Nadeem Afza

22

2013

Bushra Mubark

M. Phil MLS

HLA DQB1 * * 0201 and DQB1 0301 alleles in patients of bronchial asthma and healthy controls visiting Gulab Devi Hospital Lahore

Dr. Nadeem Afzal

23

Rakhshanda Talib

M. Phil MLS

Interlukin-6 polymorphism(634C/G) in the promotor region and the progression of diabetic nephropathy in type 2 diabetes

Dr. Nadeem Afzal

Dr. Saba Khaliq

24

Sabrina Rashid

M. Phil MLS

Determination of Neutrophil Gelainase Associated Lipocalin (NGAL) protein in patients of systemic lupus erythematous with and without renal impairment

Dr. Nadeem Afzal

25

Rabia Aslam

M. Phil MLS

Association and polymorphism of SOCS-7 gene with interferon and insulin resistance and disease progression in chronic hepatitis C

Dr. Nadeem Afzal

Dr. Saba Khaliq

26

Sana Zainab

M. Phil MLS

Determination of Rheumatoid Factor of IgG, IgM and IgA isotypes in Rheumatoid Arthritis patients

Dr. Nadeem Afzal

27

2014

Hifzah Mobeen

M. Phil MLS

Determination of antinuclear , antithyroid and anti islet cell antibodies in PCOS patients

28

Almina Ramiz

M. Phil MLS

Determination of TGF-B1 polymorphism at -509C/T promoter region in HCV and HCC patients

Dr. Shah Jahn

Dr. Nadeem Afzal

29

Adil Basir

M. Phil MLS

Polymorphism analysis of TLR 7 and TLR-9 genes in systemic lupus erythematosus

Dr. Shah Jahn

Dr. Nadeem Afzal

30

D. Hussiah Batool

M. Phil Basic

Determination of salivary il-6 and il-17 in patients of calculus associated chronic periodontitis

Dr. Nadeem Afzal

31

Dr. Ghanyia Ali

M. Phil Basic

Determination of serum NGAL level in patients of sle with and without nephritis

Dr. Nadeem Afzal

32

Dr. Ambreen Nawaz

M. Phil Basic

Determination of salivary IgA ,TGF-beta, and IL-17 in patients of dental caries

Dr. Nadeem Afzal

33

Dr. Aqsa Wajid

M. Phil Basic

Determination of anti endothelial cell, anti cardiolipin and anti glomerular antibodies in rheumatic heart disease patients

Dr. Nadeem Afzal

34

2015

Naila Irum

M. Phil MLS

Determination of TLR gene polymorphism (rs4986791) and gene expression of IFN-? and TLR-4 in hepatitis C patients.

Dr. Shah Jahan

Dr. Nadeem Afzal

35

Neelam Majeed

M. Phil MLS

Enumeration of CD3-, CD56+, CD16+ and NKG2D+ Natural Killer Cells in Breast Cancer Patients

Dr. Nadeem Afzal

Dr. Samina Khokher

36

Marwah Minhas

M. Phil MLS

Enumeration of Circulating Tumor Cells in Breast Cancer Patients

Dr. Nadeem Afzal

Dr. Samina Khokher

37

Afzal Afzal

M. Phil MLS

Determination of polymorphism +874, gene expression of IFN-? gene and serum level of IFN-? in patients of tuberculosis infections.

Dr. Shah Jahan

Dr. Nadeem Afzal

38

Dr. Wardah Irshad

M. Phil Basic

Level of urinary CD14 in patients of acute coronary syndrome.

Dr. Nadeem Afzal

39

Dr. Saima Pervaiz

M. Phil Basic

Comparison of Anti cmDNA and Anti dsDNA Antibodies in Patients of Juvenile Systemic Lupus Erythematosus

Dr. Nadeem Afzal

Dr. Asma Mushtaq

40

2016

Atia Ali

M. Phil MLS

CD14 gene (C159T) polymorphism and its surface expression on monocytes in pulmonary TB patients

Dr. Nadeem Afzal

Dr. Romeeza Tahir

41

Amna Pervaiz

M. Phil MLS

Determination of PD-L1 gene polymorphism (A/L 8923) and gene expression analysis in oropharyngeal squamous cell carcinoma

Dr. Shah Jahan

Dr. Nadeem Afzal

42

2017

Fatima Ayub

M. Phil MLS

Determination of anti-smooth muscles and anti-liver kidney microsomal type 1 autoantibodies and their association with HLA DR?1 (*04) allele in patients with chronic hepatitis C

Dr. Nadeem Afzal

Dr. Romeeza Tahir

43

2018

Dr. Attia Latif

M. Phil Basic

Determination of IL-10 gene polymorphism at promoter region (-592C/A and -1082A/G) and gene expression of IL-10 in patients of type 2 diabetes mellitus with and without nephropathy

Dr. Shah Jahan

Dr. Nadeem Afzal

44

Dr. Ghanwa Saeed

M. Phil Basic

Determination of Polymorphism in Interferon-gamma Gene (rs+874) and levels of Interferon-gamma and Myeloperoxidase in patients with Oral Lichen Planus

Dr. Romeeza Tahir

Dr. Nadeem Afzal

45

Dr. Maryam Aziz

M. Phil Basic

Levels of IL-22 in diabetes mellites type 2 patients with and without nephropathy

Dr. Nadeem Afzal

Khursheed Javaid

46

Dr. Sana Mahmood

M. Phil Basic

Serum level of CD36 in pulmonary tuberculosis and health controls

Nadeem Afzal

Dr. Faheem Shahzad

47

Dr. Sarah Samar

M. Phil Basic

Surface expression of CD40Ligand on T-cell and its level in pulmonary tuberculosis patients with or without treatment

Dr. Nadeem Afzal

Khurshee Javaid

48

Ms. Shagufta Jabeen

M. Phil MLS

Determination of Th17 cells in TB patients

Dr. Nadeem

Dr. Romeeza Tahir

49

2019

Dr. Maria Noor

M. Phil Basic

Determination of anti-Pentraxin 3 and anti-dsDNA levels in patients of SLE with and without proteinuria and in healthy conrols.

Dr. Nadeem

Khursheed Javaid

50

Dr. Aisha Kiran

M. Phil Basic

Levels of Anti-Apolipoprotein A 1 autoantibodies in patients of Rheumatoid Arthritis and health control

Dr. Romeeza Tahir

Prof. Dr. Nadeem Afzal

Dr. M. Haroon

51

Alam Khan

M. Phil MLS

Doing part-1

52

2020

Faiza Ilyas

M. Phil MLS

Doing part-1

53

Sasiha-ul-Ain

M. Phil MLS

54

Rabia Saddiqua

M. Phil MLS

55

Muhammad Asif

M. Phil MLS

56

Nimra Shahzadi

M. Phil Basic

57

Aafshar

M. Phil Basic


Ph. D Students

S#

Year

Name

Topic

Supervisor

Co-supervisor

1

2012

Dr. Nadeem Afzal

Role of genetics and immune mechanisms in the pathogenesis of diabetic retinopathy

Dr. A.H. Nagi

2

2013

Dr. Farhana Shahzad

HLA profile and immune markers in methotrexate responders and non-responders juvenile idiopathic arthritis patients

Dr. Nadeem Afzal

Dr. Romeeza Tahir

3

2015

Faheem Shahzad

Genetic variation, expression of immune response genes and cell surface markers in Mycobacterium tuberculosis infection with and without rifampicin resistance

Dr. Nadeem Afzal

Dr. Shah Jahan

4

Dr. Muhammad Kashif

Identification and Characterization of Cancer Stem Cells in Head & Neck Squamous Cell Carcinoma with and without Human Papilloma Virus

Dr. Nadeem Afzal

Dr. Saima Ch

5

2017

Khursheed Javaid

Genetic variation and expression analysis of GIMAP4, IFN-? and interlukin-17 in patients of Tuberculosis with and without Drug resistance

Dr. Nadeem Afzal

Dr. Romeeza Tahir

6

2019

Alminah Shafiq

Whole Genome Sequencing expression and gene variation analysis of SARS-CoV-2 and host genes(ACE-2, TLR, 2 and4) and immunological pattern in COVID-19 local patients

Dr. Shah Jahan

Dr. Nadeem afzal

7

Wardah Irshad

Literature Searching and synopsis writing

Dr. Romeeza tahir